Coding

Part:BBa_K3914010

Designed by: Yingxuan Gong   Group: iGEM21_SUSTech_Shenzhen   (2021-10-01)


Transcriptional repressor PhlF

Genetic programs function to integrate environmental sensors, implement signal processing algorithms and control expression dynamics[1]. The phlACBD genes responsible for the biosynthesis of the antifungal metabolite 2, 4-diacetylphloroglucinol (PHL) by the biocontrol strain Pseudomonas fluorescens F113 are regulated at the transcriptional level by the pathway-specific repressor PhlFvi[2]. Therefore, PhlF is suitable for gene circuit engineering.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 553
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 553
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 553
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 553
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 553
  • 1000
    COMPATIBLE WITH RFC[1000]



Reference

[1] Moon, T. S.; Lou, C.; Tamsir, A.; Stanton, B. C.; Voigt, C. A., Genetic programs constructed from layered logic gates in single cells. Nature 2012, 491 (7423), 249-53.

[2] Abbas, A.; Morrissey Jp Fau - Marquez, P. C.; Marquez Pc Fau - Sheehan, M. M.; Sheehan Mm Fau - Delany, I. R.; Delany Ir Fau - O'Gara, F.; O'Gara, F., Characterization of interactions between the transcriptional repressor PhlF and its binding site at the phlA promoter in Pseudomonas fluorescens F113. (0021-9193 (Print)).

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